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Multi-photon fluorescence imaging through biological tissue and image reconstruction

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conference contribution
posted on 2024-07-09, 23:44 authored by Xiaosong Gan, Min Gu
In this paper, image formation under single-photon (1-p), two-photon (2-p) and three-photon (3-p) fluorescence imaging through turbid media which consist of different sized scatterers has been investigated in detail. It has been demonstrated that the size of scattering particles plays an important role in determining whether to use 1-p, 2-p, or 3-p excitation. For small scatterers, where Rayleigh scattering is dominant, multi-photon excitation provides significantly better resolution. Such improvement reduces dramatically for large scatterers, where Mie scattering becomes dominant. Another disadvantage of using multi-photon fluorescence excitation in highly scattered media is that penetration depth is limited by fast dropping of signal strength in deep tissue imaging. In this paper, we introduce a deconvolution method with a novel concept of the effective point spread function, which is effective in restoring the loss of imaging resolution caused by multiple scattering in a tissue medium.

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ISSN

0277-786X

Journal title

Proceedings of SPIE - The International Society for Optical Engineering

Conference name

SPIE - The International Society for Optical Engineering

Volume

4431

Pagination

6 pp

Publisher

SPIE

Copyright statement

Copyright © 2001 SPIE Society of Photo-Optical Instrumentation Engineers. This paper was originally published in Proceedings of SPIE (Vol. 4431), and is available from: http://doi.org/10.1117/12.447426. The published version is reproduced in accordance with the copyright policy of the publisher. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content are prohibited.

Language

eng

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