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Fabrication of a Biocompatible Liquid Crystal Graphene Oxide–Gold Nanorods Electro- and Photoactive Interface for Cell Stimulation

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posted on 2024-07-26, 14:49 authored by Daniela Duc, Paul StoddartPaul Stoddart, Sally McArthurSally McArthur, Robert M. I. Kapsa, Anita F. Quigley, Mitchell Boyd-Moss, Simon MoultonSimon Moulton
For decades, electrode–tissue interfaces are pursued to establish electrical stimulation as a reliable means to control neuronal cells behavior. However, spreading of electrical currents in tissues limits its spatial precision. Thus, optical cues, such as near-infrared (NIR) light, are explored as alternatives. Presently, NIR stimulation requires higher energy input than electrical methods despite introduction of light absorbers, e.g., gold nanoparticles. As potential solution, NIR and electrical costimulation are proposed but with limited interfaces capable of sustaining this stimulation technique. Here, a novel electroactive nanocomposite with photoactive properties in the NIR range is constructed by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysulfosuccinimide sodium (EDC)/NHS conjugation of liquid crystal graphene oxide (LCGO) to protein-coated gold nanorods (AuNR). The liquid crystal graphene oxide–gold nanorod nanocomposite (LCGO–AuNR) is fabricated into a hydrophilic electrode-coating via drop-casting, making it appropriate for versatile electrode–tissue interface fabrication. UV–vis spectrophotometry results demonstrate that LCGO–AuNR presents an absorbance peak at 798 nm (NIR range). Cyclic voltammetry measurements further confirm its electroactive capacitive properties. Furthermore, LCGO–AuNR coating supports cell adhesion, proliferation, and differentiation of NG108-15 neuronal cells. This biocompatible interface is anticipated, with ideal electrical and optical properties for NIR and electrical costimulation, to enable further development of the technique for energy-efficient and precise neuronal cell modulation.

Funding

ARC Centre of Excellence for Electromaterials Science

Australian Research Council

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ARC Training Centre in Biodevices

Australian Research Council

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PDF (Accepted manuscript)

ISSN

2192-2659

Journal title

Advanced Healthcare Materials

Volume

8

Issue

9

Article number

1801321

Pagination

e1801321-

Publisher

Wiley

Copyright statement

Copyright © 2019. This is the peer reviewed version of the following article: Duc, D., Stoddart, P. R., McArthur, S. L., Kapsa, R. M. I., Quigley, A. F., Boyd-Moss, M., Moulton, S. E., Adv. Healthcare Mater. 2019, 8, 1801321, which has been published in final form at https://doi.org/10.1002/adhm.201801321. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Language

eng

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