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Image reconstruction through turbid media under a transmission-mode microscope

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posted on 2024-07-09, 23:44 authored by Xiaosong GanXiaosong Gan, Min Gu
In this paper, image enhancement and reconstruction through a turbid medium by utilizing polarization gating and mathematical image reconstruction methods in a microscopic imaging system are investigated. A Monte Carlo simulation model based on Mie theory and the concept of the effective point spread function (EPSF) is adopted to study image formation under a transmission-mode microscope. The results show that polarization gating methods, and particularly the differential polarization gating method, can be efficient in suppressing highly scattered light, which leads to a significant enhancement of image quality. An image reconstruction method based on the concept of the EPSF is demonstrated to be efficient in further improving image quality.

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ISSN

1083-3668

Journal title

Journal of Biomedical Optics

Volume

7

Issue

3

Pagination

5 pp

Publisher

SPIE

Copyright statement

Copyright © 2002 Society of Photo-Optical Instrumentation Engineers. This paper was originally published in the Journal of Biomedical Optics, and is available from: http://dx.doi.org/10.1117/12.780764. The published version is reproduced in accordance with the copyright policy of the publisher. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content are prohibited.

Language

eng

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