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Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

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posted on 2024-07-26, 14:01 authored by William G. A. Brown, Karina Needham, Bryony A. Nayagam, Paul StoddartPaul Stoddart
It has been demonstrated in recent years that pulsed, infrared laser light can be used to elicit electrical responses in neural tissue, independent of any further modification of the target tissue. Infrared neural stimulation has been reported in a variety of peripheral and sensory neural tissue in vivo, with particular interest shown in stimulation of neurons in the auditory nerve. However, while INS has been shown to work in these settings, the mechanism (or mechanisms) by which infrared light causes neural excitation is currently not well understood. The protocol presented here describes a whole cell patch clamp method designed to facilitate the investigation of infrared neural stimulation in cultured primary auditory neurons. By thoroughly characterizing the response of these cells to infrared laser illumination in vitro under controlled conditions, it may be possible to gain an improved understanding of the fundamental physical and biochemical processes underlying infrared neural stimulation.

Funding

Combined optical and electrical stimulation of auditory neurons

Australian Research Council

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ISSN

1940-087X

Journal title

Journal of Visualized Experiments

Volume

18

Issue

77

Article number

article no. e50444

Pagination

9 pp

Publisher

Journal of Visualized Experiments

Copyright statement

Copyright © 2013 JoVE. The published version is reproduced in accordance with the copyright policy of the publisher.

Language

eng

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