Bio-analytical screening and characterization of antioxidant compounds using online liquid chromatography techniques and mass spectrometry

This thesis is positioned in the fields of natural product and bioanalytical chemistry. It has increased the awareness and the importance of Australian native plants and mushrooms as a source of medicines. Pharmaceutical and nutraceutical industries are interested in the identification and characterization of new antioxidant compounds from natural products, which is a challenging task. In the recent years conventional online post column antioxidant assays coupled with chromatographic techniques have been used. In this study, an advanced online chromatography technique, namely Reaction Flow Chromatography is used. Untargeted metabolomics using high resolution orbitrap mass spectrometry has further explored ethnopharmacological potential of native species. All the evaluated Australian native plants were found to contain antioxidants with known therapeutic potential in cardiovascular, neurodegenerative and other chronic diseases that play a major role in the prevention/delay of oxidative stress mediated diseases.