Bioengineering 3D in-vitro neuronal cultures for biomaterial testing

This research emphasizes the importance of 3D cell cultures in studying cellular processes compared to traditional 2D cultures. While complex 3D nerve cultures often fall short in accurately representing the impact of biomaterials in the vicinity of the neurons, a new approach using the NG108-15 cell line in 3D collagen gels has been developed. This method provides a more reliable and simplified model for exploring neuron cell behaviours and interactions in 3D cultures. The study aims to contribute to drug testing and biomaterial evaluation for regenerative medicine and tissue engineering. The presented protocol is designed to assist non-biologists, such as physicists, in developing advanced imaging tools for real-time observation of 3D neuronal cultures.