Quantitative microscopy and systems biology approach to probe cellular heterogeneity in neuronal system and T cell development

This thesis investigates cellular response heterogeneity, which leads to distinct cell states, essential for understanding biological functions and diseases. Using microscopy and mathematical modeling, it explores variability in hippocampal neurons, microglia, and developing T cells. It outlines experimental protocols and modeling techniques to quantify and analyze cell states, providing insights crucial for drug development and translational research. The methodologies developed are applicable beyond the systems studied, offering a versatile toolkit for examining cellular heterogeneity in various biological contexts.